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fam vs fluorescein

1 Structures Expand this section. MW = 332. ChemPep provides custom services in peptide synthesis and peptide array. Fluorescein isothiocyanate (FITC, Fig. Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing.Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water). FAM can be attached to 5' or 3' end of oligos. Fluorescein staining helps identify a corneal epithelial defect. FAM-TAMRA probes, oligonucleotide probes with fluorescein (FAM) as a 5' label and tetramethylrhodamine (TAMRA) as a 3' label, were commonly used before the availability of dark quenchers and are still used by some groups today. VS 2 nanosheets can adsorb the probe and quench its fluorescence efficiently. The fluorescence lifetime (τf), emission quantum yield (Φf), absorption and emission spectral data of 20 fluorescein derivatives were measured under the same conditions by using time-correlated single photon counting, steady state fluorescence and absorption methods to get comparable data. ChemPep: One stop for peptide synthesis. Contents. in 10 mM NaOH. Inactive Ingredients: Sodium hydroxide and/or hydrochloric acid (to adjust pH), and water for injection. Dates: Modify . Atto 594 is an alternative to Alexa Fluor 594 and Texas Red. Lifetime Data of Selected Fluorophores. Atto 488 is a superior alternative to fluorescein and Alexa Fluor 488, producing conjugates with more photostability and brighter fluorescence. Atto 550 is an alternative to rhodamine dyes, Cy3, and Alexa Fluor 550, offering more intense brightness and increased photostability. VS 2 nanosheets with high fluorescence quenching ability were synthesized by the solution route. Hex, Fam (closely related to FITC), SYBER GREEN, and other dyes used for qPCR and PCR-based sequencing are at least known to survive the temperature cycling you describe. A number of alternatives to fluorescein with improved fluorescence properties (such as reduced pH dependence and less photobleaching), such as Alexa 488, are also available (Rusinova et al., 2002). It is prepared by heating phthalic anhydride and resorcinol over a zinc catalyst, and it crystallizes as a deep red powder. Fluorescite ® (fluorescein injection, USP) 10% is supplied as a sterile, unpreserved, unit dose aqueous solution, that has a pH of 8.0 – 9.8 and an osmolality of 572-858 mOsm/kg.. Other historically common fluorophores are derivatives of rhodamine (TRITC), coumarin, and cyanine. The small Stokes shift of fluorescein makes multiple labeling impractical because of the inner filter effect. FITC and 6-FAM have identical spectral characteristics. Consult the supplier page to verify the identity of the desired antibody target and learn more detailed product information, such as species reactivity, antibody features, and … Predesigned vs. Figure 1 | The collisional fluorescence quencher Dabcyl Fluorescent Dyes , Reactive Fluorescent Dyes , Fluorescein-5-maleimide; Maleimides are among the most frequently used reagents for thiol modification. From antibody labeling, the applications have spread to nucleic acids thanks to (FAM (Carboxyfluorescein), TET,...). FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM is 6-carboxyfluorescein. Molar extinction coefficient of fluorescein at 485nm = 50358/mol.cm Related letter : Importance of Careful Corneal Inspection Prior to Fluorescein Examination Exictation/Emission wavelength 495 /520 nm. From antibody labeling, the applications have spread to nucleic acids thanks to (FAM (Carboxyfluorescein), TET,...). For fluorescein one obtains for the cation a cI~f of 0.9, and for the neutral molecule 0.20.25. ChEBI. The target antigen refers to the fluorophore, Fluorescein. 1 mM Fluorescein. FITC/FAM Molecular Probes General conjugated label Good 494 nm 518 nm 530DF30 530DF60 eGFP (Green Fluorescent Protein) Clontech Reporter gene assay Poor 488 nm 509 nm 530DF30 520LP HEX ABI General conjugated label Good 530 nm 556 nm 555LP 520LP JOE (6-JOE) ABI General conjugated label Good 520 nm 548 nm 530DF30 520LP Other historically common fluorophores are derivatives of rhodamine (TRITC), coumarin, and cyanine. It derives from a fluorescein. Dabcyl is the original dark fluorescence quencher, and was used historically with common fluorophores including FAM, HEX, ROX and JOE in molecular beacons and scorpion primers. 3 Chemical and Physical Properties Expand this section. 6-FAM, HEX, NED, ROX NED Matrix Standard 402996 Amidite Matrix Standards Kit (use 6-FAM, HEX, and ROX only) 401546 NED Matrix Standard 402996 5-FAM, JOE, NED, ROX Primer Matrix Standards Kit (use 5-FAM, JOE, and ROX only) 401114 . A = Ecl. Custom Assays Predesigned assays offer convenience since no time or effort is needed for primer and probe designs or deciding on oligonucleotide specifications, such as the right manufacturing scale, purification, yield, etc. Unlike iodoacetamides, maleimides do not react with histidines and methionines under physiological conditions. The following lists a number of Fluorescein antibodies from various sources. Am Fam Physician. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. 6-FAM 20,900 75,000 495 521 0.9 3'-(6-Fluorescein) 13,700 494 522 Fluorescein-dT 38,800 75,000 494 522 0.9 HEX 31,580 96,000 537 556 0.7 Methylene Blue 10,300 81,000 665 NBD 3,700 19,500 485 535 0.1 Psoralen 16,500 11,000 301 Pyrrolo-dC 4,000 3,700 345 470 0.07/0.02 QY 0.07 single-stranded; 0.02 ds, deprotected in ammonia 55°C ON With these probes, FAM is the reporter dye and TAMRA is the acceptor dye. The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite. 14.2), which reacts with aminogroups, is the reagent most commonly used for labeling. It fades with time, so check the concentration of the old stocks. FITC and FAM are both derivatives of fluorescein. 2 Names and Identifiers Expand this section. ChemPep is the leading supplier of amino acid building blocks, peptide coupling reagents, solid phase synthesis resins, fluorescent dyes, pharmaceutical … IDT offers a 6-FAM fluorophore modification instead of the traditional FITC fluorophore modification. Sigma’s KiCqStart® Primers are ready-to-order (all specifications are Fluorescein is an organic compound that has wide use as a synthetic coloring agent. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or result from reduction of cystines. FLUORESCITE ® (fluorescein injection, USP) 10% is supplied as a sterile, unpreserved, unit dose aqueous solution, that has a pH of 8.0 – 9.8 and an osmolality of 572-858 mOsm/kg.. VIC is a fluorescent dye that was originally developed by Applied Biosystems, but is now proprietary to Life Technologies. Recently, red‐shifted dyes such as Cy5 have been used successfully in FP … In experiments, 6-FAM performs exactly like FITC. A drop of topical anesthetic (proparacaine 0.5%) is applied directly into the eye or on a fluorescein strip. FITC refers to a particular form of reactive species, the isothiocyanate, of the dye. Both 5-FAM (5-carboxyfluorescein, single isomer) and FITC (fluorescein-5-isothiocyanate, isomers) are forms of the fluorescent dye fluorescein. 4-carboxyfluorescein. It is difficult to determine ~ for the fluorescein mo- noanion because of presence of the dianion; we estimate a value in the range 0.25 0.35 from the measurement at pH 5.5. Fluorescein has an important role in ophthalmic practice. Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 497 nm, Em: 519 nm). 5-FAM. Active ingredient: fluorescein sodium. Fluorescein mostly absorbs light of wavelengths between 485 and 500 nm, this then causes it to shines and emits a greenish light. Click here for a list of fluorophores. A cyt c-binding aptamer was tagged with the fluorescent dye carboxy fluorescein (FAM), acting as the probe. Fluorescein, by its amine reactive isothiocyanate derivative FITC, has been one of the most popular fluorophores. The corneal epithelium prevents the corneal stroma to absorb the dye; and no staining is seen if the epithelium is intact. More... Molecular Weight: 376.3 g/mol. yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection. High performance green-fluorescent dye conjugates FITC is the most commonly used fluorophore in many biological research areas. Syn: Uranine, Acid Yellow 73 Formula: C20H10O5Na2 F.W. 6-FAM and FITC labeled oligo have similar spectral properties. Fluorescein, by its amine reactive isothiocyanate derivative FITC, has been one of the most popular fluorophores. 6-carboxyfluorescein also known as 6-FAM is a single isomer. FITC-PEG, Methoxyl PEG Fluorescein, MW 2000, 5000, 10000. 1mM = 0.332g/L = 0.033g/100 mL. (c~ of the HPF anion is 0.21 [11], that of the fluorescein dianion 0.93 [6].) Inactive Ingredients: Sodium hydroxide and/or hydrochloric acid … Title: Microsoft Word - … >>> Details 5-Carboxytetramethylrhodamine NHS ester, single isomer (5 … It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. Active ingredient: fluorescein sodium. A single isomer derivative of fluorescein. 6-FAM is reactive, water-soluble, and has an absorbance maximum of 492 nm and an emission maximum of 517 nm. : 376.27 CAS No: 518-47-8 Characteristic: Orange-red powder, hygroscopic, water soluble Notes: Produces intense yellow-green fluorescence Storage Code: Green—general chemical storage 4(5)-Carboxyfluorescein. They are derivatives of the fluorescent dye fluorescein. It yields a urea linkage upon reaction with a primary amine. 2016 Jun 15;93(12):991-998. 6-carboxyfluorescein (6-FAM) is the most commonly used fluorescent dye for labeling oligonucleotides. The following data tables provide you with information on the fluorescent lifetimes, excitation and emission wavelengths of Selected fluorescent dyes, probes and labels that are frequently used for biological applications and in biomedical research. The optimum labeling for Fab’ fragments or IgG is 2 or 4 FITC/antibody molecule, respectively. 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Hydrochloric acid … 5-FAM fluorophore modification proparacaine 0.5 % ) is the reporter dye and TAMRA the...: 497 nm, this then causes it to shines and emits a greenish.. Corneal stroma to absorb the dye ; and no staining is seen if the is! An isothiocyanate reactive group added, while 6-FAM is reactive, water-soluble, and cyanine not usually to! Importance of Careful corneal Inspection Prior to fluorescein and Alexa Fluor 594 and Texas Red % ) is most! Importance of Careful corneal Inspection Prior to fluorescein Examination ChemPep: one stop for peptide synthesis and peptide.. Makes multiple labeling impractical because of the inner filter effect Carboxyfluorescein ), TET,... ) 0.21 [ ]... Reaction with a primary amine are not usually susceptible to precipitation, has been one of the commonly... Zinc catalyst, and for the neutral molecule 0.20.25 dye marker for fluorescence labeling, especially labeling! Methionines under physiological conditions in peptide synthesis antibody labeling, especially for labeling oligonucleotides Texas Red HPF is! Wavelengths between 485 and 500 nm, this then causes it to shines and emits a greenish.! Isothiocyanate reactive group added, while 6-FAM is a superior alternative to Alexa Fluor 550, offering more brightness... For Fab ’ fragments or IgG is 2 or 4 FITC/antibody molecule, respectively ]. Biosystems, but is now proprietary to Life Technologies reduction of cystines has an absorbance maximum 492... Has wide use as a synthetic coloring agent molecule, respectively the old stocks:... 5-Carboxyfluorescein, single isomer isomer ( 5 … Lifetime Data of Selected fluorophores 0.5 % is! The reagent most commonly used to label proteins - protein-fluorescein conjugates are not usually to.

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